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axio observer 7 63x oil lens with apotome  (Carl Zeiss)


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    Carl Zeiss axio observer 7 63x oil lens with apotome
    BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 <t>40X</t> lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.
    Axio Observer 7 63x Oil Lens With Apotome, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+63x+oil+lens/apotome+microscope/pmc09986816-358-40-39
    Average 90 stars, based on 1 article reviews
    axio observer 7 63x oil lens with apotome - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release"

    Article Title: IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release

    Journal: EMBO Reports

    doi: 10.15252/embr.202255286

    BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 40X lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.
    Figure Legend Snippet: BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 40X lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.

    Techniques Used: Cell Culture, Expressing, Quantitative RT-PCR, Inhibition, Incubation, Labeling, Two Tailed Test

    Related Articles

    other:

    Article Title: IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release
    Article Snippet: EU signals were acquired on Zeiss Axio Observer 7 63X oil lens.

    Staining:

    Article Title: IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release
    Article Snippet: .. To determine the co-localization of EMCV with the endosome-lysosome network, BMDMs were treated with Syto-82-labeled-EMCV at MOI 1 for 15 min and then stained with 100 nM LysoTracker Green DND-26 (Invitrogen) for 45 min. Syto-82 signals were acquired on Zeiss Axio Observer 7 40X lens or 63X oil lens with ApoTome. .. The images were processed by using the Zeiss Zen 2012 software and quantified by ImageJ.



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    axio observer 7 63x oil lens with apotome  (Carl Zeiss)
    90
    Carl Zeiss axio observer 7 63x oil lens with apotome
    BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 <t>40X</t> lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.
    Axio Observer 7 63x Oil Lens With Apotome, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+63x+oil+lens/apotome+microscope/pmc09986816-358-40-39
    Average 90 stars, based on 1 article reviews
    axio observer 7 63x oil lens with apotome - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    axio observer 7 63x oil lens  (Carl Zeiss)
    90
    Carl Zeiss axio observer 7 63x oil lens
    BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 <t>40X</t> lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.
    Axio Observer 7 63x Oil Lens, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axio+observer+7+63x+oil+lens/63x+objective/pmc09986816__EMBR___24___e55286___s003-358-16-39
    Average 90 stars, based on 1 article reviews
    axio observer 7 63x oil lens - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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    BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 40X lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.

    Journal: EMBO Reports

    Article Title: IFN‐stimulated metabolite transporter ENT3 facilitates viral genome release

    doi: 10.15252/embr.202255286

    Figure Lengend Snippet: BMDMs were treated with different MOIs of E. coli DH5α for 30 min and further cultured for various length of time as indicated. The dynamic of ENT3 expression was determined by measuring Slc29a3 transcripts via RT‐qPCR. Results from three biological replicates ( n = 3) are shown. The efficiency of phagocytosis inhibition by Cytochalasin D treatment in macrophages. BMDMs were incubated with pHrodo Red pre‐labeled GFP‐expressing E. coli BL21 (LiveEC) at MOI 100 in the presence or absence of phagocytosis inhibitor, Cytochalasin D (CytoD), for 30 min. Images were captured by Zeiss Axio Observer 7 40X lens after washing out the extracellular E. coli . Results were representative images from three biological replicates ( n = 3). Scale bars represent 20 μm. Unpaired two‐tailed Student's t ‐test was used for statistical analysis, ** P < 0.01, ***P < 0.001, n.s. no significance. Data are shown as mean ± SEM.

    Article Snippet: To determine the co‐localization of EMCV with the endosome‐lysosome network, BMDMs were treated with Syto‐82‐labeled‐EMCV at MOI 1 for 15 min and then stained with 100 nM LysoTracker Green DND‐26 (Invitrogen) for 45 min. Syto‐82 signals were acquired on Zeiss Axio Observer 7 40X lens or 63X oil lens with ApoTome.

    Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Inhibition, Incubation, Labeling, Two Tailed Test